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bgi stereo seq kit  (Complete Genomics Inc)


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    Structured Review

    Complete Genomics Inc bgi stereo seq kit
    Spatially resolved transcriptomic analysis of two contrasting C. chinense accessions, PI 656271 and PI 660973, was performed <t>using</t> <t>Stereo-seq</t> on 5-day post-anthesis (5-dpa) fruits to investigate tissue-specific gene expression patterns. Panel A illustrates the application of spatial transcriptomics to map gene activity within intact fruit tissues. Panel B presents a Uniform Manifold Approximation and Projection (UMAP) plot of spatial transcriptomic spots, where each dot represents an individual spatial location, and colors indicate distinct transcriptional clusters across the fruit sections. Panels C and D display the differential spatial expression of AP2 (APETALA2) transcription factors and PPR (Pentatricopeptide Repeat) genes, respectively, within Modules 1 and 2—gene modules identified as highly spatially correlated and differentially expressed between the two accessions. These spatial expression profiles reveal zone-specific regulatory networks involved in the biosynthesis of capsaicinoids, carotenoids, and volatile metabolites, offering insights into the tissue-level transcriptional control of fruit quality traits in C. chinense .
    Bgi Stereo Seq Kit, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bgi stereo seq kit/product/Complete Genomics Inc
    Average 96 stars, based on 7 article reviews
    bgi stereo seq kit - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Uncovering the genetic architecture of pungency, carotenoids, and flavor in Capsicum chinense via TWAS-mGWAS integration and spatial transcriptomics"

    Article Title: Uncovering the genetic architecture of pungency, carotenoids, and flavor in Capsicum chinense via TWAS-mGWAS integration and spatial transcriptomics

    Journal: Horticulture Research

    doi: 10.1093/hr/uhaf243

    Spatially resolved transcriptomic analysis of two contrasting C. chinense accessions, PI 656271 and PI 660973, was performed using Stereo-seq on 5-day post-anthesis (5-dpa) fruits to investigate tissue-specific gene expression patterns. Panel A illustrates the application of spatial transcriptomics to map gene activity within intact fruit tissues. Panel B presents a Uniform Manifold Approximation and Projection (UMAP) plot of spatial transcriptomic spots, where each dot represents an individual spatial location, and colors indicate distinct transcriptional clusters across the fruit sections. Panels C and D display the differential spatial expression of AP2 (APETALA2) transcription factors and PPR (Pentatricopeptide Repeat) genes, respectively, within Modules 1 and 2—gene modules identified as highly spatially correlated and differentially expressed between the two accessions. These spatial expression profiles reveal zone-specific regulatory networks involved in the biosynthesis of capsaicinoids, carotenoids, and volatile metabolites, offering insights into the tissue-level transcriptional control of fruit quality traits in C. chinense .
    Figure Legend Snippet: Spatially resolved transcriptomic analysis of two contrasting C. chinense accessions, PI 656271 and PI 660973, was performed using Stereo-seq on 5-day post-anthesis (5-dpa) fruits to investigate tissue-specific gene expression patterns. Panel A illustrates the application of spatial transcriptomics to map gene activity within intact fruit tissues. Panel B presents a Uniform Manifold Approximation and Projection (UMAP) plot of spatial transcriptomic spots, where each dot represents an individual spatial location, and colors indicate distinct transcriptional clusters across the fruit sections. Panels C and D display the differential spatial expression of AP2 (APETALA2) transcription factors and PPR (Pentatricopeptide Repeat) genes, respectively, within Modules 1 and 2—gene modules identified as highly spatially correlated and differentially expressed between the two accessions. These spatial expression profiles reveal zone-specific regulatory networks involved in the biosynthesis of capsaicinoids, carotenoids, and volatile metabolites, offering insights into the tissue-level transcriptional control of fruit quality traits in C. chinense .

    Techniques Used: Gene Expression, Activity Assay, Expressing, Control



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    Image Search Results


    Spatially resolved transcriptomic analysis of two contrasting C. chinense accessions, PI 656271 and PI 660973, was performed using Stereo-seq on 5-day post-anthesis (5-dpa) fruits to investigate tissue-specific gene expression patterns. Panel A illustrates the application of spatial transcriptomics to map gene activity within intact fruit tissues. Panel B presents a Uniform Manifold Approximation and Projection (UMAP) plot of spatial transcriptomic spots, where each dot represents an individual spatial location, and colors indicate distinct transcriptional clusters across the fruit sections. Panels C and D display the differential spatial expression of AP2 (APETALA2) transcription factors and PPR (Pentatricopeptide Repeat) genes, respectively, within Modules 1 and 2—gene modules identified as highly spatially correlated and differentially expressed between the two accessions. These spatial expression profiles reveal zone-specific regulatory networks involved in the biosynthesis of capsaicinoids, carotenoids, and volatile metabolites, offering insights into the tissue-level transcriptional control of fruit quality traits in C. chinense .

    Journal: Horticulture Research

    Article Title: Uncovering the genetic architecture of pungency, carotenoids, and flavor in Capsicum chinense via TWAS-mGWAS integration and spatial transcriptomics

    doi: 10.1093/hr/uhaf243

    Figure Lengend Snippet: Spatially resolved transcriptomic analysis of two contrasting C. chinense accessions, PI 656271 and PI 660973, was performed using Stereo-seq on 5-day post-anthesis (5-dpa) fruits to investigate tissue-specific gene expression patterns. Panel A illustrates the application of spatial transcriptomics to map gene activity within intact fruit tissues. Panel B presents a Uniform Manifold Approximation and Projection (UMAP) plot of spatial transcriptomic spots, where each dot represents an individual spatial location, and colors indicate distinct transcriptional clusters across the fruit sections. Panels C and D display the differential spatial expression of AP2 (APETALA2) transcription factors and PPR (Pentatricopeptide Repeat) genes, respectively, within Modules 1 and 2—gene modules identified as highly spatially correlated and differentially expressed between the two accessions. These spatial expression profiles reveal zone-specific regulatory networks involved in the biosynthesis of capsaicinoids, carotenoids, and volatile metabolites, offering insights into the tissue-level transcriptional control of fruit quality traits in C. chinense .

    Article Snippet: Stereo-seq transcriptomics was conducted using the BGI Stereo-seq kit (catalog 211ST114, STOmics), following the manufacturer’s instructions with minor modifications [ ].

    Techniques: Gene Expression, Activity Assay, Expressing, Control